DNA Testing

How To Collect High-Quality Hair Root Samples
Collecting good hair root samples is crucial for reliable test results because bulbs are a reliable source of DNA. Proper collection will reduce the number of samples that fail in the lab due to insufficient quantity and quality of DNA.

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Getting Started
To help assure the safety of the animal and the handler, properly restrain the animal with a halter, headlock, chute or whatever is most appropriate for the animal's size. Always wash your hands or use clean gloves. If you are using a device (e.g., hemostats), be sure to wipe it clean between animals. Additional attention must be used when collecting hair roots from calves under four (4) months of age, as adequate hair roots might be difficult to obtain.

Collecting The Hair Samples

Steps:

1. Record the animal's tag number on the hair sample collector in the space provided. This animal ID will be used for reporting test results, so it's important to doublecheck for accuracy.
2. While holding the end of the tail switch, pull a pencil-thickness tuft of hair (at least 20 ' 25 hair roots) from the tail switch, making sure hair roots are attached. Roots are under the skin of the tail and extract easily when pulled correctly. Pull the hair 'up and away' from the way it lays to get as many roots as possible. Always collect dry hair samples and make sure the roots are not contaminated with feces. Hair root samples also may be obtained from other sources, such as ears or tailhead.
3. Open the hair sample collector completely. Place the hair on the back of the printed flap of the collector as shown, with roots close to the joined end. Peel off the backing paper, starting from the joined end, to expose the sticky backing of the flap.
4. Press the sticky plastic side down on top of the hair roots. Make sure the edges of the plastic are sealed around the collector. Also, verify that the roots are covered.
5. Trim the excess hair to the edges of the sample collector. Place the collectors in bundles of 10 ' 15 in a resealable plastic storage bag.
6. Record the Animal ID along with other animal information on the Zoetis Sample Information Form or the

    

   NEOGEN DNA Submission Form, such that each sample can be correctly matched with each animal. Barcodes are assigned to the samples upon arrival in the lab.
7. Send DNA sample to Beefmaster Breeders United,
   P.O. Box ,
   Bryan, TX .

How To Collect Tissue Samples

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1. Remove a TSU punch from the packaging. Punches should be assembled as one piece; if they have come apart, reassemble. Open the tube retainer at the base of the applicator by pushing the retainer clip.
2. Insert the punch into the applicator. Release the clip to lock punch into the device. Carefully squeeze the applicator handles together, guiding the punch tip into place if necessary. When the gun bolt rests flush against the red plastic clip, release the handle.
3. Remove the red plastic clip by pulling it outward. Be careful as the metal cutter above the clip is very sharp. Ensure the applicator is loaded with an unused punch (if the red plunger is visible, the punch is used and should be replaced with one that is unused).
4. Slide the applicator over the animals ear and position the metal cutter one inch from the edge of the ear, making sure to avoid any obvious veins or ridges. Squeeze handles together to take a sample and then release to free the ear. Try to do this in one swift, fluid motion.
5. Remove the punch from the applicator and check that sampling has been successful. If not, discard the sample and proceed with a new punch. Remove the used cutter from the applicator by pulling the handles apart. Discard carefully as the cutter is very sharp.
6. Record the Animal ID along with other animal information on the

    

   Zoetis Sample Information Form

    

   or the

    

   NEOGEN DNA Submission Form, such that each sample can be correctly matched with each animal. Barcodes are assigned to the samples upon arrival in the lab.
7. Send DNA sample to Beefmaster Breeders United,
   P.O. Box ,
   Bryan, TX .

DNA Testing Annoucements
Blood cards for NEOGEN are $1.50/blood card. Hair cards for NEOGEN are $2.00/hair card and a $7.00 processing fee per sample. This is an additional fee to the actual DNA test, not included. Along with blood and hair samples, we also accept tissue samples. We do have the Allflex Tissue Sampling Unit (TSUs) available in the office for purchase. The Allflex tissue applicator is $60.00. The Allflex TSU Kits are $35/per box and there are 10 punches in each box.

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